ERVFRD-1 Knockout cell line(BeWo)

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location： Home > Products > KO Cell Lines > ERVFRD-1 Knockout cell line(BeWo)

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ERVFRD-1 Knockout cell line(BeWo) Order

ERVFRD-1 Knockout cell line(BeWo)
Catalog#
YKO-H208
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ERVFRD-1 Knockout cell line(BeWo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ERVFRD-1 Knockout cell line (BeWo)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
ERVFRD-1
Gene ID
405754
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
50%DMEM/F12+40%FBS+10%DMSO
Accession
CVCL_D8ES(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript ERVFRD-1-201)
Validation: PCR+Sanger Sequencing
ERVFRD-1 Knockout cell line(BeWo) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:BeWo		 STR Info (Cell bank)
Cell Line:BEWO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 911911
D7S820 10121012
D16S539 13141314
VWA 16161616
TH01 99.399.3
AMEL XYXY
TPOX 8888
CSF1PO 11121112
D12S391 1724
FGA 222324.0
D2S1338 2021
D21S11 3030
D18S51 1416
D8S1179 1212
D3S1358 1515
D6S1043 1111
PENTAE 812
D19S433 1315
PENTAD 912
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERVFRD-1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BeWo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERVFRD-1 gene knockout ERVFRD-1 Knockout cell line (BeWo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ERVFRD-1 Knockout cell line(BeWo)
ERVFRD-1 Knockout cell line(BeWo)
Catalog#
YKO-H208
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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